koko体育app

欢迎来到《koko体育app 学报（医学版）》
蔡林奕, 孔祥丽, 谢强等. NF-κB通路在TNF-α调控软骨细胞MMPs/TIMPs比例中的作用研究[J]. koko体育app 学报（医学版）, 2016, 47(5): 642-648.
引用本文: 蔡林奕, 孔祥丽, 谢强等. NF-κB信号通路在TNF-α控制软骨组织细胞MMPs/TIMPs比率中的的作用探讨[J]. 上海专科大学学报（药学版）, 2016, 47(5): 642-648.
CAI Lin-yi, KONG Xiang-li, XIE Qiang. et alY。. The Effects of NF-kappa B Signalling on the TNF-alpha-induced Ratios of MMPs to TIMPs in Chondrocytes[J]. Journal of Sichuan University (Medical Sciences), 2016, 47(5): 642-648.
Citation: CAI Lin-yi, KONG Xiang-li, XIE Qiang. et alY。. The Effects of NF-kappa B Signalling on the TNF-alpha-induced Ratios of MMPs to TIMPs in Chondrocytes[J]. Journal of Sichuan University (Medical Sciences), 2016, 47(5): 642-648.

NF-κB通路在TNF-α调控软骨细胞MMPs/TIMPs比例中的作用研究

The Effects of NF-kappa B Signalling on the TNF-alpha-induced Ratios of MMPs to TIMPs in Chondrocytes

  • 摘要: 目的 探索核因子-κB（NF-κB）对肿瘤坏死因子-α（TNF-α）诱导下软骨细胞中基质金属蛋白酶家族（MMPs）与基质金属蛋白酶组织抑制剂家族（TIMPs）比值的影响。方法 采用胰酶消化法从1日龄小鼠的膝关节处提取软骨细胞。HE染色以显示软骨细胞形态；半定量PCR用于分析TNF-α诱导下bay11-7082对软骨细胞中MMPs和TIMPs基因表达的影响，酶谱用于鉴定TNF-α和bay11-7082作用后明胶酶活性的改变。结果 TNF-α能使MMPs和TIMPs的基因表达上调（P <0.05）。MMPs （除外MMP-1）与TIMPs 的比值均出现上调（P <0.05）。Bay11-7082能抑制TNF-α诱导下MMPs和TIMPs的表达上调，并能使MMPs/TIMPs 上调的比值下降到接近于正常软骨细胞的水平。酶谱检测明胶酶的变化，也有一致的发现。结论 TNF-α诱导下MMPs与TIMPs比值的上升可在一定程度上解释骨关节炎（OA）中软骨基质的过度降解。用bay11-7082阻断NF-κB途径可能为OA的治疗提供方案。  
    Abstract: Objective To explore the influence of the NF-κB inhibitor (bay11-7082) on tumor necrosis factor-alpha (TNF-α)-induced different ratios of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) in chondrocytes. Methods Chondrocytes were isolated from the knee joint of a 1-day old mouse by trypsin digestion method. Hematoxylin-Eosin (HE) stain was used to show the morphology of isolated chondrocytes; Semi-quantitative PCR was applied to analyze the influence of bay11-7082 on gene expressions of TNF-α-induced MMPsand TIMPsin chondrocytes; Zymography was used to elucidate activities of the gelatinases induced by TNF-α and/or bay11-7082. Results TNF-α up-regulated gene expressiosn of the MMPsand TIMPs(P<0.05). The ratios of MMPs/TIMPswere mostly increased except the part of MMP-1. Bay11-7082 could reduce TNF-α-induced MMPsand TIMPsgene expressions, and could make the increased ratio of MMPs/TIMPsdropped to the normal level of chondrocytes. Similar results were observed at the protein level of the gelatinases by zymography. Conclusion TNF-α-induced high ratios of MMPs/TIMPs could partiallyexplain over-degradation of cartilage extracellular matrix in osteoarthritis (OA). Blockage of NF-κB with bay11-7082 might provide a possible therapeutic strategy for the OA deterioration.  
    • HTML全文
© 2016 《koko体育app 学报（医学版）》编辑部　版权所有 cc

开放获取ᩚᩚᩚᩚᩚᩚ⁤⁤⁤⁤ᩚ⁤⁤⁤⁤ᩚ⁤⁤⁤⁤ᩚ𒀱ᩚᩚᩚ 本文遵循知识共享署名—非商业性使用4.0国际许可协议（CC BY-NC 4.0），允许第三方对本刊发表的论文自由共享（即在任何媒介以任何形式复制、发行原文）、演绎（即修改、转换或以原文为基础进行创作），必须给出适当的署名，提供指向本文许可协议的链接，同时标明是否对原文作了修改；不得将本文用于商业目的。CC BY-NC 4.0许可协议详情请访问

/

返回文章
返回
var _hmt = _hmt || []; (function() { var hm = document.createElement("script"); hm.src = "https://hm.baidu.com/hm.js?90c4d9819bca8c9bf01e7898dd269864"; var s = document.getElementsByTagName("script")[0]; s.parentNode.insertBefore(hm, s); })(); !function(p){"use strict";!function(t){var s=window,e=document,i=p,c="".concat("https:"===e.location.protocol?"https://":"http://","sdk.51.la/js-sdk-pro.min.js"),n=e.createElement("script"),r=e.getElementsByTagName("script")[0];n.type="text/javascript",n.setAttribute("charset","UTF-8"),n.async=!0,n.src=c,n.id="LA_COLLECT",i.d=n;var o=function(){s.LA.ids.push(i)};s.LA?s.LA.ids&&o():(s.LA=p,s.LA.ids=[],o()),r.parentNode.insertBefore(n,r)}()}({id:"K9y7iMpaU8NS42Fm",ck:"K9y7iMpaU8NS42Fm"}); koko体育-koko体育app koko体育-koko体育网页版koko体育app koko体育-全站app下载(官网) m6米乐app|下载 m6米乐app|主頁欢迎您!!