koko体育app

欢迎来到《koko体育app 学报（医学版）》
余勤, 向瑾, 石玲子, 等. 正相HPLC-MS/MS测定人血浆中多奈哌齐对映体浓度[J]. koko体育app 学报（医学版）, 2012, 43(5): 779-781,785.
引用本文: 余勤, 向瑾, 石玲子, 等. 正相HPLC-MS/MS判断人血浆中多奈哌齐对映体氧浓度[J]. 上海高中学报（临床医学版）, 2012, 43(5): 779-781,785.
YU Qin, XIANG Jin, SHI Ling-zi, et al. Determination of Donepezil Enantiomer in Human Plasma by Normal-HPLC-MS/MS[J]. Journal of Sichuan University (Medical Sciences), 2012, 43(5): 779-781,785.
Citation: YU Qin, XIANG Jin, SHI Ling-zi, et al. Determination of Donepezil Enantiomer in Human Plasma by Normal-HPLC-MS/MS[J]. Journal of Sichuan University (Medical Sciences), 2012, 43(5): 779-781,785.

正相HPLC-MS/MS测定人血浆中多奈哌齐对映体浓度

Determination of Donepezil Enantiomer in Human Plasma by Normal-HPLC-MS/MS

  • 摘要: 目的 建立正相高效液相色谱-串联质谱（HPLC-MS/MS）法测定人血浆中多奈哌齐（DN）对映体的方法，并进行方法学验证。 方法 采用正己烷：异丙醇（98：2，V/V）作为萃取剂进行样品浓集，选用CHIRALCEL OJ-H手性柱（250 mm×4.6 mm，5 μm）作分析柱，流动相为正己烷：正丙醇：二乙胺=60：40：0.1（V：V：V），流速为0.5 mL/min，柱温为38℃。以多反应监测（MRM）方式进行定量检测，利多卡因为内标，按内标法定量。 结果 左旋DN（S-DN）、右旋DN（R-DN）对映体的保留时间分别为15.56和18.41 min。S-DN的线性范围为0.051~7.596 ng/mL，R-DN的线性范围为0.049~7.404 ng/mL，线性良好（r>0.99）。S-DN和R-DN的方法回收率分别为95.10%~103.70%、93.58%~98.00%；预处理回收率分别为58.42%~61.08%、53.24%~61.87%；日内精密度（RSD）分别为8.35%~11.28%、6.78%~11.58%；日间RSD分别为5.82%~9.02%、6.87%~9.19%。 结论 建立的正相HPLC-MS/MS测定人血浆多奈哌齐对映体血药浓度具有快速、简便、灵敏、准确等优点，适用于多奈哌齐对映体的人体药动学研究。  
    Abstract: Objective To develop and validate a normal phase HPLC-MS/MS method for the determination of donepezil enantiomer in human plasma. Methods Donepezil was extracted from plasma by n-hexane:isopropanol (98:2,V/V) with lidocaine serving as an internal standard. The analytes went through the column of CHIRALCEL OJ-H (250 mm×4.6 mm, 5 μm) with mobile phase n-hexane:n-propanol:diethylamine (60:40:0.1,V/V/V). Donepezil enantiomer was determined by API 3000 in MRM mode. Results The retention time of S-DN and R-DN were 15.56 min and 18.41 min, respectively. The calibration curves were linear in a range from 0.051 to 7.596 ng/mL for S-DN, and from 0.049 to 7.404 ng/mL for R-DN, respectively, both with more than 0.99 correlation coefficients. The relative recovery were 95.10%-103.70% for S-DN and 93.58%-98.00% for R-DN, respectively; the pretreatment recovery were 58.42%-61.08% for S-DN and 53.24%-61.87% for R-DN, respectively;; the within-day RSD ranged from 8.35% to 11.28% for S-DN and from 6.78% to 11.58% for R-DN, respectively; the between-day RSD ranged from 5.82% to 9.02% for S-DN and from 6.87% to 9.19% for R-DN, respectively. Conclusion This normal phase HPLC-MS/MS method is simple, rapid, sensitive and accurate for the determination of donepezil enantiomer in human plasma and is suitable for pharmacokinetic studies of donepezil enantiomers.  
    • HTML全文
© 2012 《koko体育app 学报（医学版）》编辑部　版权所有 cc

开放获取𝔉 本文遵循知识共享署名—非商业性使用4.0国际许可协议（CC BY-NC 4.0），允许第三方对本刊发表的论文自由共享（即在任何媒介以任何形式复制、发行原文）、演绎（即修改、转换或以原文为基础进行创作），必须给出适当的署名，提供指向本文许可协议的链接，同时标明是否对原文作了修改；不得将本文用于商业目的。CC BY-NC 4.0许可协议详情请访问

/

返回文章
返回
var _hmt = _hmt || []; (function() { var hm = document.createElement("script"); hm.src = "https://hm.baidu.com/hm.js?90c4d9819bca8c9bf01e7898dd269864"; var s = document.getElementsByTagName("script")[0]; s.parentNode.insertBefore(hm, s); })(); !function(p){"use strict";!function(t){var s=window,e=document,i=p,c="".concat("https:"===e.location.protocol?"https://":"http://","sdk.51.la/js-sdk-pro.min.js"),n=e.createElement("script"),r=e.getElementsByTagName("script")[0];n.type="text/javascript",n.setAttribute("charset","UTF-8"),n.async=!0,n.src=c,n.id="LA_COLLECT",i.d=n;var o=function(){s.LA.ids.push(i)};s.LA?s.LA.ids&&o():(s.LA=p,s.LA.ids=[],o()),r.parentNode.insertBefore(n,r)}()}({id:"K9y7iMpaU8NS42Fm",ck:"K9y7iMpaU8NS42Fm"}); koko体育-koko体育app koko体育-koko体育网页版koko体育app koko体育-全站app下载(官网) m6米乐app|下载 m6米乐app|主頁欢迎您!!