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邓建军, 肖国光, 万朝敏, 等. 基因芯片技术与涂片培养技术对诊断koko体育app 结核病的比较研究[J]. koko体育app 学报（医学版）, 2013, 44(3): 429-431.
引用本文: 邓建军, 肖国光, 万朝敏, 等. 表观遗传处理器工艺应用与涂片培植工艺应用对珍断koko体育app 结核病的比设计[J]. 甘肃高中学报（中医学版）, 2013, 44(3): 429-431.
DENG Jian-jun, XIAO Guo-guang, WAN Chao-min, et al. A Comparative Study of the Gene Chip Technology and Acid-fast Staining or Mycobacterial Culture to Diagnose Childhood Tuberculosis[J]. Journal of Sichuan University (Medical Sciences), 2013, 44(3): 429-431.
Citation: DENG Jian-jun, XIAO Guo-guang, WAN Chao-min, et al. A Comparative Study of the Gene Chip Technology and Acid-fast Staining or Mycobacterial Culture to Diagnose Childhood Tuberculosis[J]. Journal of Sichuan University (Medical Sciences), 2013, 44(3): 429-431.

基因芯片技术与涂片培养技术对诊断koko体育app 结核病的比较研究

A Comparative Study of the Gene Chip Technology and Acid-fast Staining or Mycobacterial Culture to Diagnose Childhood Tuberculosis

  • 摘要: 目的  探讨结核杆菌DNA微阵列芯片技术用于诊断koko体育app 结核病的临床应用价值。 方法  采集住院koko体育app 的70份临床诊断结核病及50份非结核病患儿的标本,每份标本分别进行抗酸染色、分枝杆菌培养以及结核杆菌DNA微阵列芯片检测,比较DNA微阵列芯片检测结果与抗酸染色镜检和分枝杆菌培养的结果。 结果  DNA微阵列芯片检测结核病患儿分枝杆菌的敏感性为24.3%(17/70),涂片抗酸染色敏感性为17.1%(12/70),分枝杆菌培养敏感性为20.0%(14/70),3种方法特异性均为100.0%(50/50)。DNA微阵列芯片检测结果与抗酸染色或分枝杆菌培养结果之间差异无统计学意义。 结论  DNA微阵列芯片技术对诊断koko体育app 结核病有一定参考价值,为koko体育app 结核病的诊断提供了新思路。  
    Abstract: Objective  To investigate the clinical value of mycobacterial DNA microarray technology for diagnosis of childhood tuberculosis.   Methods  120 clinical specimens were collected from hospitalized child patients. Acid-fast staining, mycobacterial culture and DNA microarray assays were performed using these clinical specimens. The results of DNA microarray assays were compared with the results of acid-fast staining and mycobacterial culture.   Results  The sensitivity of DNA microarray assays for specimens from children with tuberculosis was 24.3% (17/70), of acid-fast staining 17.1% (12/70), of mycobacterial culture 20.0% (14/70), and the specificity of the three methods was all 100.0% (50/50). The difference between results of DNA microarray assays and that of acid-fast staining or mycobacterial culture was not significant.   Conclusion  DNA microarray assay has reference value for the diagnosis of childhood tuberculosis. It provides a new way for the diagnosis of childhood tuberculosis.  
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