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张翔, 厉红元, 闵捷等. 乳腺癌细胞中下调RABEX-5对其化疗敏感性的影响[J]. koko体育app 学报（医学版）, 2013, 44(6): 882-885.
引用本文: 张翔, 厉红元, 闵捷等. 乳线癌生殖细胞中调整RABEX-5对其放疗敏非理性性的后果[J]. 广东高校学报（医疗版）, 2013, 44(6): 882-885.
ZHANG Xiang, LI Hong-yuan, MIN Jie. et al. The Effect of RABEX-5 Downregulation on the Chemosensitivity of Human Breast Cancer Cells[J]. Journal of Sichuan University (Medical Sciences), 2013, 44(6): 882-885.
Citation: ZHANG Xiang, LI Hong-yuan, MIN Jie. et al. The Effect of RABEX-5 Downregulation on the Chemosensitivity of Human Breast Cancer Cells[J]. Journal of Sichuan University (Medical Sciences), 2013, 44(6): 882-885.

乳腺癌细胞中下调RABEX-5对其化疗敏感性的影响

The Effect of RABEX-5 Downregulation on the Chemosensitivity of Human Breast Cancer Cells

  • 摘要: 目的 探讨RABEX-5下调表达后，乳腺癌细胞对蒽环类药物和紫杉类药物的敏感性变化。方法 构建RABEX-5 RNAi慢病毒表达载体,并感染人乳腺癌细胞株MCF-7，使细胞内RABEX-5基因表达沉默， Real-time PCR、Western blot方法检测沉默效应。应用CCK-8方法检测RABEX-5沉默后（MCF-7/RNAi），与其阴性对照人乳腺癌细胞株（MCF-7/vector）相比对化疗药物敏感性的变化情况。结果 RABEX-5 RNAi慢病毒表达载体感染MCF-7后，与未干扰组（MCF-7）及阴性对照组(MCF-7/vector)相比，RABEX-5的mRNA和蛋白水平表达下调。与阴性对照组相比，RABEX-5下调表达后，对蒽环类药物表柔比星的化疗敏感性降低,半数致死浓度(IC50)分别为（3.590 0±0.228 69） μg/mL、（1.193 3±0.187 71） μg/mL，差异有统计学意义（P50)分别为（11.162 7±0.210 26） μg/mL、（10.536 7±0.430 97） μg/mL，差异无统计学意义（P>0.05）。结论 RABEX-5下调表达使人乳腺癌细胞MCF-7对蒽环类药物表柔比星产生耐药，而对紫杉类药物多西他赛敏感，为进一步研究RABEX-5在乳腺癌个体化治疗中的作用提供理论基础。  
    Abstract: Objective To investigate the changes in sensitivity to anthracycline and taxanes of human breast cancer cells after RABEX-5 downregulated. Methods By constructing a lentiviral vector for RNA interference (RNAi) of RABEX-5 gene and transfected into human breast cancer cell line MCF-7 to silence the express of RABEX-5. Real-time PCR and Western blot were used to detect the silencing effect. The changes in sensitivity to chemotherapeutic drugs of MCF-7/RNAi and its negative control cell lines MCF-7/vector were detected by CCK-8 reagent. Results Compared with MCF-7 cell and MCF-7/vector cell, the expression of RABEX-5 mRNA and protein was downregulated in MCF-7/RNAi cells. The sensitivity to epirubicin was reduced after downregulating the expression of RABEX-5, the 50% inhibition concentration (IC50) of MCF-7/RNAi 〔(3.590 0±0.228 69) μg/mL〕 was higher than that of MCF-7/Vvector 〔(1.193 3±0.187 71) μg/mL, P50 were (11.162 7±0.210 26) μg/mL and (10.536 7±0.430 97) μg/mL, respectively (P>0.05). Conclusion Downregulation of RABEX-5 can induce chemoresistance to epirubicin in human breast cancer cell MCF-7; while its effect on sensitivity to docetaxel is not significant. This study can provide a theoretical basis for future research RABEX-5 in the individualized treatment of breast cancer.  
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