HA/PMMA纳米复合暂冠材料的合成及其性能表征和细胞相容性检测
Characteristics of Hydroxyapatite/PMMA Nanocomposites for Provisional Restoration and Its Biocompatibility withHuman Gingival Fibroblasts
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摘要: 目的 合成具有良好生物相容性的羟基磷灰石 (HA)/聚甲基丙烯酸甲酯 (PMMA) (H/P)复合暂时冠材料。 方法 将一定量的纳米HA与PMMA混合,分别合成HA质量分数为20% (20 wt%)、30% (30 wt%)、40% (40 wt%)、50% (50 wt%)的4组复合材料。此复合材料经固化、打磨、抛光,扫描电镜 (SEM)观察其表面微结构。将人牙龈成纤维细胞接种在以上各组及纯PMMA组、纯钛组样本表面,共同培养24 h后,免疫荧光标记细胞骨架;培养1、3、5、7 d后,MTT法测细胞活力。 结果 SEM结果显示,20 wt% H/P组和30 wt% H/P组的纳米HA团聚体基本被PMMA基质所覆盖,40 wt% H/P组样本表面的团聚体尺寸小而均匀,直径约为2~4 μm,而在50 wt% H/P组表面,团聚体直径达到5~10 μm,表面可见较多裂隙。40 wt% H/P组和50 wt% H/P组细胞铺展成长梭形,其他H/P组及纯PMMA组细胞较圆缩。40 wt% H/P组各时点细胞活力与纯钛组的差异无统计学意义 (P>0.05);其余H/P组及纯PMMA组细胞活力多低于纯钛组,差异有统计学意义 (P<0.05)。 结论 40 wt%H/P生物相容性接近纯钛,有望用于种植修复暂时冠材料,改善软组织美学。Abstract: Objective To formulate hydroxyapatite(HA)/polymethyl methacrylate (PMMA) composites with improved cytocompatibility for provisional restoration. Methods Nanocomposites with 20 wt%,30 wt%,40 wt%,and 50 wt% HA/PMMA (H/P) were developed and examined using X-ray photoelectron spectroscopy (XPS) and scanning electron microscope (SEM). Human gingival fibroblasts were cultured on those HA/PMMA discs and investigated by fluorescent staining on 24 h and MTT assay at 1 d, 3 d, 5 d and 7 d. Results Chemical integration of HA/PMMA interface was confirmed by XPS. Typical fusiform cells with adhesion spots were detected on 40 wt% and 50 wt% H/P discs. MTT results showed insignificant differences in cell growth between 40 wt% H/P and pure titanium (Ti,P>0.05), while the other H/P discs showed significantly lower cell growth than pure Ti (P<0.05). Conclusion 40 wt% H/P might be a promising candidate for provisional dental implant restoration and for esthetic gingival contour.
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