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洪涛, 崔路可, 文集等. 虫草素对补体复合物介导的足细胞损伤的保护作用[J]. koko体育app 学报（医学版）, 2015, 46(2): 173-178.
引用本文: 洪涛, 崔路可, 文集等. 虫草素对补体分手后复合物介导的足组织损害的庇护目的[J]. 重庆院校学报（医美版）, 2015, 46(2): 173-178.
HONG Tao, CUI Lu-ke, WEN Ji. et al. Cordycepin Protects Podocytes from Injury Mediated by Complements Complex C5b-9[J]. Journal of Sichuan University (Medical Sciences), 2015, 46(2): 173-178.
Citation: HONG Tao, CUI Lu-ke, WEN Ji. et al. Cordycepin Protects Podocytes from Injury Mediated by Complements Complex C5b-9[J]. Journal of Sichuan University (Medical Sciences), 2015, 46(2): 173-178.

虫草素对补体复合物介导的足细胞损伤的保护作用

Cordycepin Protects Podocytes from Injury Mediated by Complements Complex C5b-9

  • 摘要: 目的 探讨冬虫夏草的生物活性单体成分——虫草素（3′-脱氧腺苷）对补体介导的足细胞损伤的保护作用及其可能机制。方法 补体复合物C5-9介导的足细胞损伤作为膜性肾病的体外模型。采用永生化的小鼠足细胞株MPC5建立细胞损伤模型，虫草素作为干预药物。用电镜观察细胞超微形态结构的变化，用免疫荧光染色观察细胞骨架F-actin结构和足细胞特异蛋白nephrin的表达，用Western blot检测信号通路丝裂原活化蛋白激酶（MAPK）磷酸化水平。结果 C5b-9刺激细胞3 h后，细胞二级足突消失，细胞骨架结构明显损伤，F-actin应力纤维混乱，nephrin分布由胞膜移至胞浆，并引起p38、JNK、ERK磷酸化。虫草素能够保护足细胞的足突和细胞骨架结构，抑制nephrin 的重分布，并显著抑制补体介导的p38/JNK 信号通路活化。结论 虫草素能保护足细胞对抗补体介导的损伤，其机制至少部分是通过抑制p38/JNK信号通路活化而实现的。  
    Abstract: Objective To explore the protective effect of Cordycepin (3’-deoxyadenosine), a bioactive compound of Cordyceps Sinensis, on injury of podocytes. Methods C5b-9-induced podocyte injury was used as a model of membranous nephropathy \in vitro. This model was established using mouse podocyte cell line——MPC5. Cordycepin was given as an intervention. Ultra-micro morphological changes were observed by electron microscope. F-actin cytoskeleton and expression of nephrin were observed by fluorescence microscope. The phosphorylation of mitogen-activated protein kinase (MAPK) was measured by Western blot. Results Stimulated by C5b-9 for 3 h, MPC5 showed secondary foot processes, with cytoskeleton structure damaged, nephrin relocated from the cell surface to the cytoplasm, and cell signal pathway-p38, JNK and ERK activated. Cordycepin protected foot processes and cytoskeleton structures of podocytes, suppressed the redistribution of nephrin, and inhibited p38/JNK action. Conclusion Cordycepin can protect podocyte from C5b-9-induced injury partly through inhibiting the activation of p38/JNK signaling pathway.  
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