koko体育app

欢迎来到《koko体育app 学报（医学版）》
孔令恒, 孙娜, 朱娟霞, 等. 脊髓NMDA受体和NK1受体在大鼠心脏伤害性感受信息传递中的作用[J]. koko体育app 学报（医学版）, 2013, 44(5): 722-726.
引用本文: 孔令恒, 孙娜, 朱娟霞, 等. 脊髓NMDA多巴胺蛋白激酶和NK1多巴胺蛋白激酶在大鼠灵魂输出有魅力受企业信息引入中的用[J]. 广东综合大学学报（中医药学版）, 2013, 44(5): 722-726.
KONG Ling-heng, SUN Na, ZHU Juan-xia, et al. The Role of NMDA and NK1 Receptors in Cardiac Nociceptive Information Transmissions in the Spinal Cords of Rats[J]. Journal of Sichuan University (Medical Sciences), 2013, 44(5): 722-726.
Citation: KONG Ling-heng, SUN Na, ZHU Juan-xia, et al. The Role of NMDA and NK1 Receptors in Cardiac Nociceptive Information Transmissions in the Spinal Cords of Rats[J]. Journal of Sichuan University (Medical Sciences), 2013, 44(5): 722-726.

脊髓NMDA受体和NK1受体在大鼠心脏伤害性感受信息传递中的作用

The Role of NMDA and NK1 Receptors in Cardiac Nociceptive Information Transmissions in the Spinal Cords of Rats

  • 摘要: 目的 观测大鼠脊髓鞘内分别注射NMDA受体或NK1受体的激动剂和拮抗剂后,对心包腔内注射辣椒素(CAP)诱发的背斜方肌肌电图(EMG)的影响,以探索心脏伤害性感受信息在脊髓内传递的突触机制。 方法 将雄性SD大鼠随机分为7组:对照组、NMDA组、MK801组、MK801+NMDA组、Sar-SP组、CP-96345组和CP-96345+Sar-SP组。通过在大鼠心包腔内注射CAP,建立大鼠心脏痛模型;实验动物分别行心包内插管术和脊髓鞘内置管术,记录并观察鞘内注射药物后心包内注射CAP诱发的EMG的变化。 结果 ①对照组大鼠鞘内注射生理盐水后,心包内注射CAP所诱发的背斜方肌放电个数(96.9%±12.5%)与鞘内注射前CAP所诱发的放电个数(前对照,100%)相比没有明显改变(P>0.05)。②鞘内注射NMDA受体激动剂NMDA后,背斜方肌放电数(185.2%±24.4%)与前对照相比增加,差异有统计学意义(P<0.05);鞘内给予NMDA受体拮抗剂MK801后,大鼠背斜方肌肌电与前对照相比,差异无统计学意义(P>0.05);鞘内给予NMDA受体拮抗剂MK801+激动剂NMDA,背斜方肌放电个数(106.6%±10.2%)与前对照相比有所增加,但差异没有统计学意义(P>0.05)。③鞘内注射NK1受体激动剂Sar-SP,背斜方肌放电个数(145.6%±10.1%)与前对照比较增加,差异有统计学意义(P<0.05);鞘内注射NK1受体拮抗剂CP-96345,背斜方肌放电未见明显改变(P>0.05);鞘内注射NK1受体拮抗剂CP-96345+激动剂Sar-SP,背斜方肌放电数(102.2%±8.4%)与前对照相比有所增加,但差异没有统计学意义(P>0.05)。 结论 脊髓NMDA受体和NK1受体都可能参与了心脏伤害性感受信息在脊髓内的传递。  
    Abstract: Objective To determine the effects of NMDA and NK1 receptor agonist and antagonist on the EMG and the synaptic mechanism of nociceptive information transmissions in the spinal cords. Methods Male SD rats were randomly divided into seven groups, with intrathecal injection of the following chemicals respectively:control group (10 μL saline), NMDA group (0.147 μg/10 μL NMDA), MK801 group (6.8 μg/10 μL MK801), MK801+NMDA group (6.8 μg/10 μL MK801+0.147 μg/10 μL NMDA), Sar-SP group (1.4 μg/10 μL Sar-SP), CP-96345 group (5 μg/10 μL CP-96345), and CP-96345+Sar-SP group (1.4 μg/10 μL Sar-SP+5 μg/10 μL CP-96345). A cardiac pain model in rats through intrapericardial injection of capsaicin was established. Intrapericardial injection of capsaicin was given to the rats 10 min after intrathecal injection of the tested chemicals. The spinotrapezius electromyography (EMG) activities as an index of cardiac-somatic motor reflex were recorded simultaneously. Results Compared with the pre-test controls (100%), saline did not make a significant change to the capsaicin-evoked EMG response (96.9%±12.5%, P>0.05); NMDA agonist increased the capsaicin-evoked EMG response (185.2%±24.4%) significantly (P<0.05); neither MK801 nor a combined administration of MK801 and NMDA made a significant change to the capsaicin-evoked EMG response (106.6%±10.2%, P>0.05); Sar-SP increased the capsaicin-evoked EMG response (145.6%±10.1%) significantly (P<0.05); whereas neither CP-96345 nor a combined administration of CP-96345 and Sar-SP made a significant change to the capsaicin-evoked EMG response (102.2%±8.4%, P>0.05). Conclusion NMDA and NK1 receptors may have participated in the transmissions of cardiac nociception information in the spinal cords of rats.  
    • HTML全文
© 2013 《koko体育app 学报（医学版）》编辑部　版权所有 cc

开放获取🌺 本文遵循知识共享署名—非商业性使用4.0国际许可协议（CC BY-NC 4.0），允许第三方对本刊发表的论文自由共享（即在任何媒介以任何形式复制、发行原文）、演绎（即修改、转换或以原文为基础进行创作），必须给出适当的署名，提供指向本文许可协议的链接，同时标明是否对原文作了修改；不得将本文用于商业目的。CC BY-NC 4.0许可协议详情请访问

/

返回文章
返回
var _hmt = _hmt || []; (function() { var hm = document.createElement("script"); hm.src = "https://hm.baidu.com/hm.js?90c4d9819bca8c9bf01e7898dd269864"; var s = document.getElementsByTagName("script")[0]; s.parentNode.insertBefore(hm, s); })(); !function(p){"use strict";!function(t){var s=window,e=document,i=p,c="".concat("https:"===e.location.protocol?"https://":"http://","sdk.51.la/js-sdk-pro.min.js"),n=e.createElement("script"),r=e.getElementsByTagName("script")[0];n.type="text/javascript",n.setAttribute("charset","UTF-8"),n.async=!0,n.src=c,n.id="LA_COLLECT",i.d=n;var o=function(){s.LA.ids.push(i)};s.LA?s.LA.ids&&o():(s.LA=p,s.LA.ids=[],o()),r.parentNode.insertBefore(n,r)}()}({id:"K9y7iMpaU8NS42Fm",ck:"K9y7iMpaU8NS42Fm"}); koko体育-koko体育app koko体育-koko体育网页版koko体育app koko体育-全站app下载(官网) m6米乐app|下载 m6米乐app|主頁欢迎您!!