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脂质体姜黄素在Lewis肺癌中的抗肿瘤和抗血管作用
Liposomal Curcumin Inhibits Tumor Growth and Angiogenesis in Lewis Lung Cancer
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摘要: 目的 制备水溶性的脂质体姜黄素,研究其抗肿瘤和抗血管生成的作用。 方法 采用乙醇注入法制备脂质体姜黄素。MTT法检测脂质体姜黄素对小鼠肺癌细胞LL/2 的抑制作用,流式细胞术检测脂质体姜黄素对细胞周期和细胞凋亡的影响。建立小鼠Lewis肺癌模型,检测脂质体姜黄素的抗肿瘤作用。采用藻酸盐实验检测脂质体姜黄素的抗血管生成作用。 结果 在体外,脂质体姜黄素可以抑制小鼠肺癌细胞LL/2的增殖,阻滞细胞周期,并引起细胞凋亡;在体内,脂质体姜黄素抑制了小鼠Lewis肿瘤的生长,藻酸盐实验验证了脂质体姜黄素能抑制肿瘤内的血管生成。 结论 脂质体姜黄素能抑制LL/2细胞的增殖并诱导细胞凋亡,通过静脉给药能有效抑制Lewis肺癌在小鼠体内的生长。Abstract: Objective To prepare water soluble curcumin liposome and investigate its anti-tumour and anti-angiogenic effects. Methods Liposomal curcumin was prepared by alcohol injection method. Proliferation inhibition to murin Lewis lung cancer cell line LL/2 of curcumin liposome was evaluated by MTT assay. Apoptosis and cell cycle arrest induced by liposomal curcumin were analysed by flow cytometry. Anti-tumour effects were investigated in a murine lung cancer model, and the anti-angiogenic effect was determined by aginate encapsulation assay. Results In vitro, liposomal curcumin inhibits the proliferation of LL/2 cells and induces apoptosis and cell cycle arrest. In vivo, the systemic administration of liposomal curcumin resulted in the inhibition of tumour. Aginate encapsulation assay revealed angiogenesis was decreased by curcumin liposome. Conclusion The curcumin liposome treatment can significantly inhibit tumour growth in vivo.
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