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李星斓, 陈雪芹, 聂玲, 等. 抗凋亡基因Bfl-1在前列腺癌中的表达及作用<[J]. koko体育app 学报（医学版）, 2013, 44(1): 21-26.
引用本文: 李星斓, 陈雪芹, 聂玲, 等. 抗凋亡基因Bfl-1在前列腺癌中的表达及作用<[J]. koko体育app 学报（医学版）, 2013, 44(1): 21-26.
LI Xing-lan, CHEN Xue-qin, NIE Ling, et al. The Expression and Function of Anti-apoptotic Bfl-1 in Prostate Cancer[J]. Journal of Sichuan University (Medical Sciences), 2013, 44(1): 21-26.
Citation: LI Xing-lan, CHEN Xue-qin, NIE Ling, et al. The Expression and Function of Anti-apoptotic Bfl💫-1 in Prostate Cancer[J]. Journal of Sichuan University (Medical Sciences), 2013, 44(1): 21-26.

抗凋亡基因Bfl-1在前列腺癌中的表达及作用<

The Expression and Function of Anti-apoptotic Bfl-1 in Prostate Cancer

  • 摘要: 目的 检测Bcl2相关蛋白A1(Bfl-1) mRNA在前列腺癌细胞株、前列腺癌组织及良性前列腺增生组织中的表达,观测阻断Bfl-1表达对前列腺癌细胞的影响,探讨Bfl-1在前列腺癌发生发展中的作用。 方法 采用RT-PCR、实时荧光定量PCR(Q-PCR)、cDNA探针原位杂交(ISH)等技术检测Bfl-1 mRNA在前列腺癌组织、细胞株和良性前列腺增生组织的表达水平;结合临床资料分析Bfl-1的表达与临床病理指标的关系;利用反义寡核苷酸干预前列腺癌细胞株,RT-PCR检测Bfl-1表达,MTT法检测细胞的存活,倒置显微镜下观察前列腺癌细胞的形态变化。 结果 RT-PCR、Q-PCR结果显示Bfl-1 mRNA在激素非依赖性前列腺癌细胞株PC-3和DU145中高表达,在激素依赖性前列腺癌细胞株LNCaP中未见表达,表达差异有统计学意义(P<0.05);原位杂交结果显示Bfl-1 mRNA在前列腺癌组织中高表达,而在良性前列腺增生组织中未见表达,表达差异有统计学意义(P<0.05);有转移、分期高的前列腺癌病例的Bfl-1 mRNA表达水平高于无转移、分期低的病例(P<0.05);Bfl-1反义寡核苷酸转染PC-3和DU145细胞后, Bfl-1表达下调,细胞生长受到抑制(P<0.05),细胞脱落、碎裂形成大小不一的细胞团块。 结论 激素非依赖性前列腺癌细胞的生长与Bfl-1过表达有关;干预Bfl-1表达可能成为晚期前列腺癌治疗的新策略。  
    Abstract: Objective To determine the expression of Bcl2 related protein A1(Bfl-1) mRNA in prostate cancer cell lines and tissues, and to explore the functions of Bfl-1 in prostate adenocarcinoma. Methods RT-PCR, real-time quantitative PCR(Q-PCR)and in situ hybridization (ISH) were used to detect the expression of Bfl-1 mRNA in prostate cancer cell lines, tissues and benign prostate hyperplasia (BPH) tissue samples. The relationship between Bfl-1 expression and clinicopathological parameters was analyzed. Antisense oligonucleotides (ASONs) were used to interfere the expression of Bfl-1 and its effects on prostate cancer cells. MTT was used to detect the survival, morphologic changes of prostate cancer cells was observed by inverted microscope. Results Bfl-1 mRNA, detected by RT-PCR, Q-PCR and ISH, was overexpressed in the androgen-independent prostate cancer cell lines PC-3 and DU145, but not detectable in the androgen-dependent prostate cancer cell line LNCaP and BPH tissue samples (P<0.05). Significantly higher Bfl-1 mRNA levels were observed in higher stage and metastatic prostate cancer cases than those without metastasis or of low stage. ASONs targeting Bfl-1 significantly inhibited androgen-independent prostate cancer cell growth (P<0.05), cell was rounding off or fragmentation. Conclusion Bfl-1 is involved in maintaining the hormone-independent prostate cancer cell growth. Bfl-1 may become a new therapeutic target in advanced prostate cancer.  
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