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唐梅, 王琪, 李赫冬等. 胰岛素样生长因子-Ⅰ在神经前体细胞中抗凋亡的作用[J]. koko体育app 学报（医学版）, 2014, 45(1): 15-18.
引用本文: 唐梅, 王琪, 李赫冬等. 胰岛素样发展神经元-Ⅰ在神经末梢前体神经元中抗凋亡的效应[J]. 甘肃大学专业学报（分子生物学版）, 2014, 45(1): 15-18.
TANG Mei, WANG-qi, LI He-dong. et al. Study on the Anti-apoptosis Effect of Insulin-like Growth FactorⅠon the Regulation of Neural Precursor Cells Survival[J]. Journal of Sichuan University (Medical Sciences), 2014, 45(1): 15-18.
Citation: TANG Mei, WANG-qi, LI He-dong. et al. Study on the Anti-apoptosis Effect of Insulin-like Growth FactorⅠon the Regulation of Neural Precursor Cells Survival[J]. Journal of Sichuan University (Medical Sciences), 2014, 45(1): 15-18.

胰岛素样生长因子-Ⅰ在神经前体细胞中抗凋亡的作用

Study on the Anti-apoptosis Effect of Insulin-like Growth FactorⅠon the Regulation of Neural Precursor Cells Survival

  • 摘要: 目的 探讨胰岛素样生长因子-I（insulin-like growth factor I, IGF-I）对神经前体细胞抗凋亡的影响。方法 以大鼠神经前体细胞（即Ge6细胞）为研究对象，体外培养Ge6细胞，在培养基中加入含IGF-I 10 ng/mL为实验组，不加IGF-I为对照组，分别继续培养2 d、4 d、6 d，定量实时逆转录聚合酶链式反应（qRT-PCR）检测IGF-I对Ge6细胞caspase-3 mRNA表达的影响。采用细胞免疫荧光染色观察各组细胞caspase-3蛋白的阳性表达率。结果 qRT-PCR结果显示，实验组Ge6细胞caspase-3 mRNA的表达较对照组低，实验组培养4 d（2.007±0.297）和6 d（1.194±0.244）与对照组培养4 d（5.530±1.027）和6 d（5.371±0.513）的差异有统计学意义（P均<0.05）。细胞荧光染色表明培养2 d、4 d、6 d实验组Ge6细胞caspase-3蛋白的阳性表达率较对照组减少，实验组培养4 d（3.5%±0.2%）和6 d（5.1%±0.1%）与对照组培养4 d（7.0%±0.5%）和6 d（7.4%±0.4%）caspase-3蛋白的阳性表达率的差异有统计学意义（P均<0.05）。结论 IGF-I对神经前体细胞具有抗凋亡作用。  
    Abstract: Objective?To investigate the effect of insulin-like growth factor Ⅰ (IGF-Ⅰ) on the anti-apoptosis of neural precursor cells. Methods?Rat neural precursor cells (Ge6) were used as experiment object. 10 ng/mL IGF-Ⅰ were applied to cultured Ge6 differentiated 2 d, 4 d, 6 d and the untreated cells as control. Quantitative reverse transcriptase PCR (qRT-PCR) was used to detect the effect of the IGF-Ⅰ on caspase-3 mRNA in the Ge6 of different groups and Immunofluorescent staining were applied to obverse the positive rate of caspase-3. Results?qRT-PCR demonstrated that caspase-3 mRNA level of experimental group decreased compared to that of the control group, the differences between experimental group cultured for 4 d (2.007±0.297), 6 d (1.194±0.244) and the control group cultured for 4 d (5.530±1.027), 6 d (5.371±0.513) were statistically significant (P<0.05). Immunofluorescent staining indicated that the caspase-3 positive rate of experimental group cultured for 2 d, 4 d and 6 d were reduced compared to that of the control group, the differences between experimental group cultured for 4 d (3.5%±0.2%), 6 d (5.1%±0.1%) and the control group cultured for 4 d (7.0%±0.5%), 6 d (7.4%±0.4%) were statistically significant (P<0.05). Conclusion?IGF-I plays an anti-apoptosis role on neural precursor cells.  
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