胸腺肽α1对小鼠机体免疫系统的影响

李炜晔; 陆惠敏; 郭强等

胸腺肽α1对小鼠机体免疫系统的影响

Effects of Thymosin α1 on Immune Effector Molecules of Mouse

摘要

摘要: 目的探讨胸腺肽α1（Tα1）对正常小鼠机体相关免疫效应分子的作用及胸腺肽α1诱导特异性免疫耐受的机制。方法将BABL/c小鼠分为4组，正常对照组（无任何药物，n=5)、环孢素A（CsA）组（给予10 mg/kg CsA,n=20）、Tα1组（给予400 μg/kg Tα1,n=20）、CsA+Tα1组(CsA、Tα1剂量同前，n=20)，每日腹腔注射1次，共14 d。分别于用药后第1、7、14、21 d各取5只（正常对照组取1只）采血取血清行液相芯片细胞因子（反映Th1、Th2和Th17功能）检测。处死小鼠后取脾脏，制备小鼠脾细胞悬液进行淋巴细胞培养，仍分为4组，除对照组外，其余3组分别加入0.25 mg/mL CsA、10 μg/mL Tα1、0.25 mg/mL CsA+10 μg/mL Tα1，培养3 d后检测细胞的生长增殖，以及培养上清液细胞因子的表达。结果 BABL/c小鼠给予Tα1、CsA后，与正常对照组比较，白介素（IL）-1a、IL-2、IL-6、IL-17等炎性细胞因子降低（P<0.05），而抑炎因子IL-10升高（P<0.05），CsA和Tα1对细胞因子表达的影响相似（不同时间点及不同细胞因子两单药组间差异均无统计学意义），CsA和Tα1联合用药可加强单用药作用。淋巴细胞培养3 d后，Tα1、CsA、CsA+Tα1组淋巴细胞增殖受抑制，与正常对照组比较，差异有统计学意义；培养细胞上清液细胞因子表达与正常对照组比较，各组IL-1α、IL-6表达下降，IL-10升高，差异均有统计学意义（P<0.05），而IL-2、IL-17各组间差异无统计学意义。结论 Tα1对小鼠正常机体免疫功能具有一定的调节作用，促使T细胞由Th1向Th2转化。

Abstract: Objective To analysis the effects of Tα1 on the immune effector molecules in mouse immune system. Methods Sixty five BABL/c mice were divided into four groups: CsA group (n=20), Tα1 group (n=20), CsA+Tα1 group (n=20) and control group (n=5). In the 3 experimental groups, 10 mg/kg CsA, 400 μg/kg Tα1, 10 mg/kg CsA+400 μg/kg Tα1 were respectively administrated by intraperitoneal injection daily. Luminex was performed for cytokine detection at 1 d, 7 d, 14 d, 21 d day after the above treatments. Lymphocyte culture was prepared with the mouse spleen suspension, and then treated with 0.25 mg/mL CsA, 10 μg/mL Tα1 or 0.25 mg/mL CsA+10 μg/mL Tα1 in vitro, respectively. Three days later, OD values of each treated lymphocyte culture and several cytokines in the culture were measured. Results Compared with other groups, CsA+Tα1 group had significant lower IL-1α, IL-2, IL-6, IL-17, and significant higher IL-10 at 1 d, 7 d, 14 d, 21 d after the treatments （P＜0.05）. Three days after the culture, OD value in the control group was significantly higher than that in Tα1 group, CsA group, and CsA+Tα1 group (P<0.05). IL-1α and IL-6 in the control group were significantly higher than those in the experiment groups (P<0.05), while IL-10 in the control group was significantly lower than that in the experiment groups (P<0.05). IL-2 and IL-17 were similar. Conclusion Tα1 show regulatory effect on the immune effector molecules which could promote Th1 cells transforming to Th2 cells.

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