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张菊梅, 周建业, 薄磊等. 牙周炎牙根根面微生物群落结构比较[J]. koko体育app 学报（医学版）, 2016, 47(6): 848-851.
引用本文: 张菊梅, 周建业, 薄磊等. 牙体炎牙齿根面微分子生物学群落架构是比较[J]. 安徽师范大学学报（临床版）, 2016, 47(6): 848-851.
ZHANG Ju-mei, ZHOU Jian-ye, BO Lei. et al. Microbial Community Structure on the Root Surface of Patients with Periodontitis[J]. Journal of Sichuan University (Medical Sciences), 2016, 47(6): 848-851.
Citation: ZHANG Ju-mei, ZHOU Jian-ye, BO Lei. et al. Microbial Community Structure on the Root Surface of Patients with Periodontitis[J]. Journal of Sichuan University (Medical Sciences), 2016, 47(6): 848-851.

牙周炎牙根根面微生物群落结构比较

Microbial Community Structure on the Root Surface of Patients with Periodontitis

  • 摘要: 目的 利用高通量测序，研究牙周炎患牙根面微生物的群落结构，为牙周炎的进展研究提供实验依据。方法 选取同一牙周炎患者的6颗Ⅲ度松动患牙，分别刮取根颈（RN组）、根中（RM组）、根尖（RT组）菌斑及组织，利用Illumina Miseq 测序平台，对16S rRNA V3V4区进行双端测序；通过Mothur、Qiime和 SPSS等软件分析根面不同部位微生物的群落结构及差异。结果 主坐标分析（PCoA）显示，RM与RT组微生物群落结构较接近，但二者与RN组有明显区别；3组共检出13个菌门，优势菌门（丰度>1%）共7个；184种菌属中检出优势菌属（丰度>1%）29个，其中拟杆菌门_［G-6］及消化链球菌科_［XI］［G-4］丰度会随着采样部位的深入而增加（P<0.05），而普雷沃菌属、月形单胞菌属、棒状杆菌属及欧森氏菌属的丰度随着采样部位的深入而减少（P<0.05）。结论 牙周炎患牙根面微生物群落结构具有一定的部位特异性。  
    Abstract: Objective To study the microbial community structure on the root surface of patients with periodontitis. Methods Bacterial plaque and tissues from the root neck （RN group）, root middle （RM group） and root tine （RT group） of six teeth with mobility Ⅲ in one patient with periodontitis were sampled. The V3V4 region of 16S rRNA was sequenced on the Illumina MiSeq platform. The microbial community structure was analyzed by Mothur, Qiime and SPSS software. Results The principal component analysis (PCoA) results indicated that the RM samples had a similar microbial community structure as that of the RT samples, which was significant different from that of the RN samples. Thirteen phyla were detected in the three groups of samples, which included 7 dominant phyla. 29 dominant genera were detected in 184 genera. The abundance of Bacteroidetes_［G-6］ and Peptostre ptococcaceae_［XI］［G-4］ had a positive correlation with the depth of the collection site of samples (P<0.05), while the abundance of Prevotella, Selenomonas, Corynebacterium and Olsenella had a negative correlation with the depth of the collection site of samples (P<0.05). Conclusion There is region-specificity of microbial community structure on the root surface of patients with periodontitis.  
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