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刘流, 刘琳, 梁力川等. IL-1β预处理脂肪间充质干细胞促进VEGF蛋白分泌和巨噬细胞M2型极化的研究[J]. koko体育app 学报（医学版）, 2019, 50(2): 171-176.
引用本文: 刘流, 刘琳, 梁力川等. IL-1β预处理脂肪间充质干细胞促进VEGF蛋白分泌和巨噬细胞M2型极化的研究[J]. koko体育app 学报（医学版）, 2019, 50(2): 171-176.
LIU Liu, LIU Lin, LIANG Li-chuan.et al. Effects of IL-1β Pretreated Adipose Tissue-derived Mesenchymal Stem Cells on VEGF Secretion and Macrophage M2 Polarization[J]. Journal of Sichuan University (Medical Sciences), 2019, 50(2): 171-176.
Citation: LIU Liu, LIU Lin, LIANG Li-chuan.et al. Effects of IL-1β Pretreated Adipose Tissue-derived Mesenchymal Stem Cells on VEGF Secretion and Macrophage M2🌞 Polarization[J]. Journal of Sichuan University (Medical Sciences), 2019, 50(2): 171-176.

IL-1β预处理脂肪间充质干细胞促进VEGF蛋白分泌和巨噬细胞M2型极化的研究

Effects of IL-1β Pretreated Adipose Tissue-derived Mesenchymal Stem Cells on VEGF Secretion and Macrophage M2 Polarization

  • 摘要: 目的探讨白细胞介素（IL）-1β预处理脂肪间充质干细胞（ADMSCs）对血管内皮生长因子(VEGF)蛋白分泌和巨噬细胞M2型极化的影响及作用机制。方法IL-1β预处理ADMSCs 24 h，Western blot法检测ADMSCs中环氧合酶-2（COX-2）蛋白表达，ELISA法测定ADMSCs培养基中前列腺素E2(PGE2)和VEGF蛋白质量浓度; IL-1β预处理的ADMSCs细胞培养基培养丙二醇甲醚醋酸酯（PMA）-U937巨噬细胞（M0巨噬细胞）72 h后，实时荧光定量PCR法检测PMA-U937细胞CD163（巨噬细胞M2型极化标志物）和IL-10 mRNA表达。慢病毒干扰ADMSCs细胞中COX-2表达后，观察ADMSCs培养基中VEGF蛋白质量浓度（ELISA法）和PMA-U937巨噬细胞中CD163和IL-10 mRNA的表达（实时荧光定量PCR法）。结果IL-1β预处理ADMSCs细胞后上调其COX-2蛋白表达，增加PGE2和VEGF蛋白分泌（P<0.05）；IL-1β预处理的ADMSCs细胞培养基可上调PMA-U937巨噬细胞中CD163和IL-10 mRNA表达水平（P<0.01）。干扰ADMSCs细胞中COX-2蛋白表达后，可抑制IL-1β预处理的ADMSCs细胞中VEGF蛋白分泌（P<0.05），亦可抑制其对PMA-U937巨噬细胞中CD163和IL-10 mRNA表达的影响（P<0.05）。结论IL-1β预处理ADMSCs细胞可通过COX-2-PGE2信号轴，增加VEGF蛋白分泌，以及诱导PMA-U937巨噬细胞M2型极化，提示IL-1β预处理能够增强ADMSCs细胞抗炎活性。  
    Abstract: ObjectiveTo investigate the effects of interleukin (IL)-1β preteated adipose tissue-derived mesenchymal stem cells (ADMSCs) on vascular endothelial growth factor (VEGF) secretion and macrophages M2 polarization. MethodsAfter IL-1β pretreated ADMSC for 24 h,the expression of cyclooxygenase-2 (COX-2) was detected using Western blot,and the secretions of prostaglandin E2 (PGE2) and VEGF were measured by ELISA method. Conditioned media collected from ADMSCs was used for culturing PMA-U937 macrophages for 72 h,and the expressions of CD163 and IL-10 mRNAs were detected using qRT-PCR. After inhibition of COX-2 expression in ADMSCs by Lentivirus silencing,secretion of VEGF and CD163 and IL-10 mRNA expressions were detected. ResultsIL-1β pretreating ADMSCs increased the expression level of COX-2,enhanced PGE2 and VEGF secretions (P<0.05). In addition,the mRNA levels of CD163 and IL-10 were upregulated when ADMSCs were pretreated by IL-1β in PMA-U937 cells. After downregulation of COX-2 in ADMSCs,the secretion of VEGF was suppressed,and the mRNA levels of CD163 and IL-10 were also significantly down-regulated. ConclusionIL-1β pretreatment enhanced the secretion of PGE2 and VEGF,and induced macrophage M2 polarization,which depended on COX-2-PGE2 signal pathway.  
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