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黄丽萍, 陈晨, 王雪萍等. 5-Aza-CdR对子宫内膜癌中RASSF1A基因甲基化的作用[J]. koko体育app 学报（医学版）, 2015, 46(3): 380-383.
引用本文: 黄丽萍, 陈晨, 王雪萍等. 5-Aza-CdR对联宫腔内膜癌中RASSF1A表观遗传甲基化的的作用[J]. 北京社会学报（医学研究版）, 2015, 46(3): 380-383.
HUANG Li-ping, CHEN Chen, WANG Xue-ping. et al. The Role of 5-Aza-CdR on Methylation of Promoter in RASSF1A Gene in Endometrial Carcinoma[J]. Journal of Sichuan University (Medical Sciences), 2015, 46(3): 380-383.
Citation: HUANG Li-ping, CHEN Chen, WANG Xue-ping. et al. The Role of 5-Aza-CdR on Methylation of Promoter in RASSF1A Gene in Endometrial Carcinoma[J]. Journal of Sichuan University (Medical Sciences), 2015, 46(3): 380-383.

5-Aza-CdR对子宫内膜癌中RASSF1A基因甲基化的作用

The Role of 5-Aza-CdR on Methylation of Promoter in RASSF1A Gene in Endometrial Carcinoma

  • 摘要: 目的 探讨去甲基化药物5-氮杂-2’-脱氧脱苷（5-Aza-CdR）对子宫内膜癌中Ras相关区域家族1A（RASSF1A）基因甲基化的作用。方法 取子宫内膜癌细胞株HEC-1-B细胞随机分组，用不同浓度（1.0×10-6，3.0×10-6，10.0×10-6 mol/L)的去甲基化药物5-Aza-CdR处理后，采用甲基化特异性PCR、实时定量PCR、Western blot、TUNEL等技术，分析空白对照组及实验各组子宫内膜癌细胞株HEC-1-B中RASSF1A基因启动子区域甲基化情况、mRNA表达水平、蛋白表达和细胞凋亡的情况。结果 人子宫内膜癌细胞株HEC-1-B 细胞中RASSF1A基因启动子区存在高甲基化,mRNA、蛋白低表达，以及细胞凋亡失控。不同浓度的5-Aza-CdR可逆转RASSF1A基因高甲基化状态，恢复mRNA、蛋白表达量，并可抑制HEC-1-B 细胞的生长及诱导凋亡。结论 子宫内膜癌异常甲基化RASSF1A基因作为治疗靶点，去甲基化药物5-Aza-CdR是基因治疗的有效途径。  
    Abstract: Objective To explore the effect of demethylating drug 5-Aza-2′-deoxycytidine (5-Aza-CdR) on methtylation status of the Ras-association domain family1A gene (RASSF1A) in human endometrial carcinoma. Methods Randomly assign the human endometrial carcinoma cell line HEC-1-B into groups and use demethylating drug 5-Aza-CdR of different concentration to treat them. Then Methylation-specific polymerase chain reaction (MSP), real-time PCR, Western blot, TUNEL technology were used to analyze methylation status of RASSF1A promoter CpG islands,RASSF1A mRNA expression, RASSF1A protein expression and apoptosis of HEC-1-B cell. Results High DNA methylation in RASSF1A gene promoter region, low RASSF1A mRNA level and protein expression and out of control of human endometrial carcinoma cell HEC-1-B apoptosis were observed. 5-Aza-CdR of different concentration could reverse RASSF1A gene’s methylation status, recover the expression of mRNA and protein, and control the growth of HEC-1-B by inducing apoptosis. Conclusion Aberrant methylation of RASSF1A in endometrial cancer as a therapeutic target, demethylating agent 5-Aza-CdR could be an effective way of gene therapy.  
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