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廖凤, 但齐琴, 杜润芬等. 脑缺血肺损伤大鼠肺组织TNF-α的表达变化[J]. koko体育app 学报(医学版), 2012, 43(6): 914-918.
引用本文: 廖凤, 但齐琴, 杜润芬等. 脑坏死肺挫伤大鼠肺团队TNF-α的表达方式变动[J]. 杭州一本大学学报(医学研究版), 2012, 43(6): 914-918.
LIAO Feng, DAN Qi-qin, DU Run-fen. et al. Expression of TNF-α in Lung Tissue of Rats with Lung Injury Induced by Brain Ischemia[J]. Journal of Sichuan University (Medical Sciences), 2012, 43(6): 914-918.
Citation: LIAO Feng, DAN Qi-qin, DU Run-fen. et al. Expression of TNF-α in Lung Tissue of Rats with Lung Injury Induced by Brain Ischemia[J]. Journal of Sichuan University (Medical Sciences), 2012, 43(6): 914-918.

脑缺血肺损伤大鼠肺组织TNF-α的表达变化

Expression of TNF-α in Lung Tissue of Rats with Lung Injury Induced by Brain Ischemia

  • 摘要: 目的 探讨脑缺血后损伤肺组织肿瘤坏死因子-α(TNF-α)的表达变化。方法 成年SD大鼠随机分为假手术组、脑缺血肺损伤组(n=13),其中每组5只用于免疫组化检测TNF-α在肺的定位分布,余8只分别用于RT-PCR和Western blot检测TNF-α mRNA和蛋白水平变化。术后24 h取肺组织行RT-PCR、术后48 h取肺组织行Western blot检测肺组织TNF-α mRNA和蛋白表达水平;术后3 d取肺组织行免疫组织化学染色检测肺组织内TNF-α的定位分布。结果 肺组织TNF-α mRNA和蛋白表达在脑缺血肺组织明显增加,与假手术组比较,差异有统计学意义(P<0.05)。TNF-α主要分布在气道上皮细胞和巨噬样细胞。结论 脑缺血后损伤肺组织TNF-α表达上调,提示其可能与脑缺血肺损伤炎症反应有关。  
    Abstract: 【Abstract】 Objective To investigate the expression of tumor necrosis factor-α (TNF-α) in the lung tissue of rats with lung injury induced by brain ischemia. Methods The rat model of lung injury induced by brain ischemia was established. At 24 h, 48 h, 72 h after brain ischemia, lung tissues were harvested from each rats, the expressions of TNF-α mRNA and protein and its distributions in the lung tissue were measured by the methods of RT-PCR (n=8), Western blot (n=8), and immunohistochemistry (n=5), respectively. Results The increase of TNF-α mRNA and protein levels were found in the lung tissues after brain ischemia, when compared with sham group (P<0.05). The morphological study with immunohistochemical staining observed TNF-α positive reaction in epithelial cells and some macrophages in the lung tissues after brain ischemia. Conclusion The expression of TNF-α in the lung tissue could be upregulated in the rats with brain ischemia.  
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