koko体育app

欢迎来到《koko体育app 学报(医学版)》
杨芸, 荣荣, 但齐琴等. BDNF抗体封闭对大鼠脑缺血损伤肺组织IL-6表达的影响[J]. koko体育app 学报(医学版), 2012, 43(6): 922-926.
引用本文: 杨芸, 荣荣, 但齐琴等. BDNF表面抗原封闭型对大鼠脑缺血性挫裂伤肺聚集IL-6表答的印象[J]. 云南综合大学学报(中医药学版), 2012, 43(6): 922-926.
YANG Yun, RONG Rong, DAN Qi-qin. et al. Effects of BDNF Antibody Block on IL-6 Expression in Lung of Rats Subjected to Brain Ischemia[J]. Journal of Sichuan University (Medical Sciences), 2012, 43(6): 922-926.
Citation: YANG Yun, RONG Rong, DAN Qi-qin. et al. Effects of BDNF Antibody Block on IL-6 Expression in Lung of Rats Subjected to Brain Ischemia[J]. Journal of Sichuan University (Medical Sciences), 2012, 43(6): 922-926.

BDNF抗体封闭对大鼠脑缺血损伤肺组织IL-6表达的影响

Effects of BDNF Antibody Block on IL-6 Expression in Lung of Rats Subjected to Brain Ischemia

  • 摘要: 目的 探讨脑缺血肺损伤后脑源性神经营养因子(BDNF)是否能调节损伤肺组织炎症因子白细胞介素-6(IL-6)的表达。方法 将大鼠随机分为假手术组、脑缺血肺损伤组、脑缺血肺损伤给予BDNF抗体封闭组(BDNF抗体封闭组)。每组13只大鼠,于BDNF抗体干预后3 d处死,5只用于免疫组化,8只用于RT-PCR和Western blot,检测BDNF封闭对IL-6定位与表达量的影响。结果 免疫组化染色显示,气道上皮细胞和巨噬细胞可见IL-6表达,胞浆染色为主。RT-PCR和Western blot检测显示BDNF抗体封闭后3 d,肺组织IL-6 mRNA和蛋白表达下降,与脑缺血肺损伤组比较,差异有统计学意义(P<0.05)。 结论 BDNF抗体封闭减少脑缺血肺组织IL-6表达,提示BDNF可能通过调节IL-6参与脑缺血肺水肿。  
    Abstract: 【Abstract】 Objective To explore effect of brain-derived neurotrophic factor (BDNF) on IL-6 expression in the lung of rats with brain ischemia. Methods Inflammatory lung injury was induced by brain ischemia in rats that were devided into sham operation group, brain ischemia lung injury (BILI) group and brain ischemia with BDNF administration group. Lungs were harvested from rats in each group in 3 days after brain ischemia respectively. Immunohistochemistry, RT-PCR and Western blot were employed to detect the expressional changes on the mRNA and protein of IL-6 in the lung tissues. Results Expression of IL-6 was mainly found in cytoplasm of lung epithelial cell and macrophage with immunohistochemistry staining. A significant decrease of IL-6 expression was observed at 3 days after brain ischemia with BDNF antibody block. Conclusion BDNF, as a crucial neurotrophic factor, could regulate IL-6 expression in rats lung tissues with brain ischemia.  
© 2012 《koko体育app 学报(医学版)》编辑部 版权所有 cc

开放获取♒ 本文遵循知识共享署名—非商业性使用4.0国际许可协议(CC BY-NC 4.0),允许第三方对本刊发表的论文自由共享(即在任何媒介以任何形式复制、发行原文)、演绎(即修改、转换或以原文为基础进行创作),必须给出适当的署名,提供指向本文许可协议的链接,同时标明是否对原文作了修改;不得将本文用于商业目的。CC BY-NC 4.0许可协议详情请访问

/

返回文章
返回
var _hmt = _hmt || []; (function() { var hm = document.createElement("script"); hm.src = "https://hm.baidu.com/hm.js?90c4d9819bca8c9bf01e7898dd269864"; var s = document.getElementsByTagName("script")[0]; s.parentNode.insertBefore(hm, s); })(); !function(p){"use strict";!function(t){var s=window,e=document,i=p,c="".concat("https:"===e.location.protocol?"https://":"http://","sdk.51.la/js-sdk-pro.min.js"),n=e.createElement("script"),r=e.getElementsByTagName("script")[0];n.type="text/javascript",n.setAttribute("charset","UTF-8"),n.async=!0,n.src=c,n.id="LA_COLLECT",i.d=n;var o=function(){s.LA.ids.push(i)};s.LA?s.LA.ids&&o():(s.LA=p,s.LA.ids=[],o()),r.parentNode.insertBefore(n,r)}()}({id:"K9y7iMpaU8NS42Fm",ck:"K9y7iMpaU8NS42Fm"}); koko体育-koko体育app koko体育-koko体育网页版koko体育app koko体育-全站app下载(官网) m6米乐app|下载 m6米乐app|主頁欢迎您!!