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雍莉, 王宇, 邹晓莉等. HPLC/MS/MS同时测定血浆肾上腺素、去甲肾上腺素、可的松和氢化可的松的方法建立[J]. koko体育app 学报(医学版), 2014, 45(1): 142-146.
引用本文: 雍莉, 王宇, 邹晓莉等. HPLC/MS/MS时候旋光度的测定血浆肾上腺素、去甲肾上腺素、可的松和氢化可的松的方式 制定[J]. 江苏大家学报(医药学版), 2014, 45(1): 142-146.
YONG Li, WANG Yu, ZOU Xiao-li. et al. Simultaneous Determination of Adrenaline, Noradrenaline, Cortisone and Cortisol in Plasma with HPLC/MS/MS[J]. Journal of Sichuan University (Medical Sciences), 2014, 45(1): 142-146.
Citation: YONG Li, WANG Yu, ZOU Xiao-li. et al. Simultaneous Determination of Adrenaline, Noradrenaline, Cortisone and Cortisol in Plasma with HPLC/MS/MS[J]. Journal of Sichuan University (Medical Sciences), 2014, 45(1): 142-146.

HPLC/MS/MS同时测定血浆肾上腺素、去甲肾上腺素、可的松和氢化可的松的方法建立

Simultaneous Determination of Adrenaline, Noradrenaline, Cortisone and Cortisol in Plasma with HPLC/MS/MS

  • 摘要: 目的 建立高效液相色谱-质谱-质谱联用 (HPLC/MS/MS)同时测定血浆中的肾上腺素、去甲肾上腺素、可的松和氢化可的松的方法。方法 样品经乙腈沉淀蛋白和萃取后,15 000 r/min 离心5 min,取上清液进样分析。电喷雾离子源 (ESI), 正离子检测, 多反应监测 (MRM) 方式定量分析。 结果 肾上腺素、去甲肾上腺素、可的松和氢化可的松在0.02~200.00 ng/mL浓度范围内线性良好 (相关系数均≥0.999),检出限分别为4.13 pg/mL、4.64 pg/mL、4.29 pg/mL和4.52 pg/mL,日内和日间精密度分别为1.19%~5.42%和2.16%~6.04%;将所建立的方法用于空白人血浆样品分析,加标回收率为80.0%~109.0%,样品测定精密度为3.93%~7.57%。结论 本研究所建立的方法快速灵敏,适合血浆样品的批量分析。  
    Abstract: Objective?To develop a method for simultaneous determination of adrenaline, noradrenaline, cortisone and cortisol in plasma using HPLC/MS/MS. Methods?Sample proteins were precipitated with acetonitrile and the sample solution was injected into HPLC/MS/MS after centrifugation at 15 000 r/min for 5 min. Electrospray ionization (ESI) and the positive ion detection were applied with a multiple reaction monitoring (MRM) mode for quantitative analyses. Results?Under the optimal conditions, good linearity (r>0.999) was observed in the range of 0.02-200.00 ng/mL of target compounds. The detection limit reached 4.13 pg/mL, 4.64 pg/mL, 4.29 pg/mL and 4.52 pg/mL for adrenaline, noradrenaline, cortisone and cortisol respectively. The inter-day and intra-day precisions ranged from 1.19%-5.42% and 2.16%-6.04% respectively. Satisfied results were achieved using human plasma samples, with a spiked recovery in the range of 80.0%-109.0% and a relative standard deviation of 3.93%-7.57%. Conclusion?The proposed method is quick, sensitive and suitable for batch analyses of plasma samples.  
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