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互作转录组测序枝术在生物工程科学研究中的应该用进展情况

许萌萌 孙雨婷 陈虹 杨德琴

许萌萌, 孙雨婷, 陈虹, 等. 互作转录组测序技术在微生物研究中的应用进展[J]. koko体育app 学报(医学版), 2022, 53(2): 201-207. doi: 10.12182/20220360203
引用本文: 许萌萌, 孙雨婷, 陈虹, 等. 互作转录组测序技术在微生物研究中的应用进展[J]. koko体育app 学报(医学版), 2022, 53(2): 201-207. doi:
XU Meng-meng, SUN Yu-ting, CHEN Hong, et al. Application Progress of Dual RNA Sequencing in Microbial Research[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCE EDITION), 2022, 53(2): 201-207. doi: 10.12182/20220360203
Citation: XU Meng-meng, SUN Yu-ting, CHEN Hong, et al. Application Progress of Dual RNA Sequencing in Microbial Research[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCE EDITION), 2022, 53(2): 201-207. doi:

互作转录组测序技术在微生物研究中的应用进展

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基金项目: 国家自然科学基金面上项目(No. 31970783)、重庆市医学重点学科建设项目(2011年55号)和重庆市博士后研究项目特别资助(渝人社办[2020]379号)资助
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    E-mail: yangdeqin@hospital.cqmu.edu.cn

Application Progress of Dual RNA Sequencing in Microbial Research

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  • 摘要: 人体内定植着数量庞大、结构复杂的微生物群,微生物群之间、微生物群及宿主细胞间存在着复杂的相互作用。互作转录组测序技术(dual RNA sequencing, Dual RNA-seq)可同时分析两个(或多个)互作物种间基因表达的动态变化,并通过互作模型图获得物种间基因的调控关系,得到物种间的相互作用机制。本文就近年来Dual RNA-seq技术在肠道、呼吸道、皮肤及口腔微生物研究中的应用现状及发展前景作一综述。自Dual RNA-seq的概念被引入以来,该技术已被应用于一系列感染模型。在分子水平上直接研究物种间动态相互作用,有助于更好地理解感染过程中病原体和宿主的生理变化,从而揭示潜在的新靶点或生物标志物。但是,Dual RNA-seq目前仍然处于起步阶段,在实验技术方面存在一定的局限性,比如由于物种间的相互作用是动态变化的,最佳实验条件以及采样点的选择以及如何实现实时观测是亟需解决的难题;此外,由于Dual RNA-seq的生物信息学数据量大,如何快速灵活地处理互作信息仍然需要进一步的探索。
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出版历程
  • 收稿日期:  2022-08-27
  • 修回日期:  2022-12-15
  • 刊出日期:  2023-03-22

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