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以SPF级早产猪树立肺發育对模型的系统化实践科研

王秋,  周丹,  谢亮,  石芳,  张伟坚,  伏洪玲,  刘瀚旻,  丁玉春

王秋, 周丹, 谢亮, 等. 以SPF级早产猪建立肺发育模型的初步实验研究[J]. koko体育app 学报（医学版）, 2021, 52(5): 789-793. doi: 10.12182/20210960504
引用本文: 王秋, 周丹, 谢亮, 等. 以SPF级早产猪建立肺发育模型的初步实验研究[J]. koko体育app 学报（医学版）, 2021, 52(5): 789-793. doi:
WANG Qiu, ZHOU Dan, XIE Liang, et al. Preliminary Experimental Study of Establishing a Lung Development Model with Specific Pathogen Free Preterm Pigs[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCE EDITION), 2021, 52(5): 789-793. doi: 10.12182/20210960504
Citation: WANG Qiu, ZHOU Dan, XIE Liang, et al. Preliminary Experimental Study of Establishing a Lung Development Model with Specific Pathogen Free Preterm Pigs[J]. JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCE EDITION), 2021, 52(5): 789-793. doi:

以SPF级早产猪建立肺发育模型的初步实验研究

doi: 
  • 1. 重庆一本大学华西第一大医院 治愈医疗科 （成都市 610041）
  • 2. 甘肃高校时华西然后诊所/清华高校时我的生命学科实训基地 肺成长与重要性的疾病综合实验英文室 （程度 610041）
  • 3. 河北二本大学华西第2医疗 孩子透气免疫性科 （武汉 610041）
  • 4. 降生问题与涉及到妇儿疾患教学部重心调查室（江苏大专） （绵阳 610041）
  • 5. 部委清洁营养协会会精力生物制品学突出检测室（甘肃一本大学） （合肥 610041）
  • 6. 南京市畜牧局科学研究院 （南京 402460）
基金项目: 国家自然科学基金（No. 81941007）和四川省科技计划重点研发项目（No. 2019YFS0037）资助
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    E-mail：hanmin@vip.163.com

Preliminary Experimental Study of Establishing a Lung Development Model with Specific Pathogen Free Preterm Pigs

  • 1. Department of Rehabilitation Medicine, West China Second University Hospital, Sichuan University, Chengdu 610041, China
  • 2. The Joint Laboratory for Lung Development and Related Diseases of West China Second University Hospital, Sichuan University and School of Life Sciences of Fudan University, Chengdu 610041, China
  • 3. Department of Pediatric Pulmonology and Immunology, West China Second University Hospital, Sichuan University, Chengdu 610041, China
  • 4. Key Laboratory of Birth Defects and Related Diseases of Women and Children of the Ministry of Education, Sichuan University, Chengdu 610041, China
  • 5. Key Laboratory of Chronobiology of the National Health Commission, Sichuan University, Chengdu 610041, China
  • 6. Chongqing Academy of Animal Sciences, Chongqing 402460, China
More Information
  • 摘要:   目的  探究在排除高氧、机械通气、药物等医学干预的条件下，建立无特定病原体（specific pathogen free, SPF）级巴马早产猪肺发育动物模型的最佳剖宫产时间。  方法  选择胎龄113 天（gestational days, GD）、GD107、GD104、GD101和GD98的SPF级巴马猪系母猪分别进行剖宫产，观察早产仔猪存活情况。根据早产猪的一般资料、存活情况和肺石蜡切片HE染色观察结果，寻找最佳的建立SPF级早产猪肺发育模型的剖宫产时间。  结果  剖宫产7只母猪，共获取仔猪55只，其中产后3 h仍存活的共25只。GD98和GD101的仔猪全部死亡，GD104的仔猪存活7只，存活率为33.33%（7/21），GD107和GD113的仔猪全部存活。GD98的仔猪已具备完整的外形和肺部形状；GD104仔猪的肺部开放度已较大，薄壁型肺泡密度更高；GD107仔猪的肺基本处于开放状态，薄壁肺泡密度基本与正常足月猪相同。  结论  本研究提示可以利用无医疗干预的GD104 SPF级早产猪建立SPF级早产猪肺发育模型。
    Abstract:   Objective  To determine the best time for conducting cesarean section for the establishment of an animal model of lung development with specific pathogen free (SPF) preterm Bama minipigs under the condition of not making medical interventions such as hyperoxia, mechanical ventilation, or medication.  Methods  SPF Bama sows at gestational day (GD) 113, GD107, GD104, GD101, and GD98 were selected and cesarean sections were performed. Then, the viability of the preterm piglets were observed. Based on their general data, viability, and paraffin sections stained with hematoxylin and eosin, the best time for performing cesarean section in order to build a SPF preterm pig model of lung development was determined.  Results  Cesarean sections were performed on a total of 7 sows and 55 piglets were delivered, among which 25 were still alive 3 hours after delivery. Seven piglets of GD104 and all piglets of GD107 and GD113 survived, while piglets of GD98 and GD101 all died. The survival rate of piglets of GD104 was 33.33% (7/21). Piglets of GD98 already possessed fully developed physical appearance and lung shape. Piglets from GD104 had better lung expansion and higher density of thin-walled alveoli. The lungs of GD107 piglets were basically fully expanded, and the density of thin-walled alveoli was almost the same as that of normal full-term piglets.  Conclusions  Findings of this study suggest that SPF preterm piglets of GD104 with no specific pathogen exposure and no medical intervention can be used to establish a SPF preterm pig model of lung development.
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    图  1  GD98和GD113仔猪的肺部外形

    Figure  1.  ඣ External appearance of the lungs of GD98 and GD113 piglets

    图  2  不同胎龄肺组织在低倍镜下的观察结果。HE染色 ×40

    Figure  2.  ꧋ Observation of lung tissue of different gestational ages at low magnification. HE staining ×40

    A: GD98; B: GD101; C: GD104; D:GD107; E: GD113.

    图  3  不同胎龄肺组织在高倍镜下的观察结果。HE染色 ×200

    Figure  3.  ♓ Observation of lung tissue of different gestational ages at high magnification. HE staining ×200

    A: GD98; B: GD101; C: GD104; D:GD107; E: GD113.

    表  1  各组仔猪一般情况

    Table  1.   🌳 General information of the piglets in each group

    GroupnLive piglets/case (%)Body mass/gGender (male/female)
    GD98 5 0 (0) 240±29.15 1/4
    GD101 12 0 (0) 234±53.51 4/8
    GD104 21 7 (33.3) 391±90.91 9/12
    GD107 5 5 (100) 546±51.77 2/3
    GD113 12 12 (100) 615±101.22 8/4
    　GD: Age in gestational days.
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出版历程
  • 收稿日期:  2022-05-12
  • 修回日期:  2022-08-12
  • 网络出版日期:  2022-12-06
  • 刊出日期:  2022-09-20

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