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王秋, 周丹, 谢亮, 等. 以SPF级早产猪建立肺发育模型的初步实验研究[J]. koko体育app 学报(医学版), 2021, 52(5): 789-793. DOI:
引用本文: 王秋, 周丹, 谢亮, 等. 以SPF级早产猪建立肺发育模型的初步实验研究[J]. koko体育app 学报(医学版), 2021, 52(5): 789-793. DOI:
WANG Qiu, ZHOU Dan, XIE Liang, et al. Preliminary Experimental Study of Establishing a Lung Development Model with Specific Pathogen Free Preterm Pigs[J]. Journal of Sichuan University (Medical Sciences), 2021, 52(5): 789-793. DOI:
Citation: ౠ WANG Qiu, ZHOU Dan, XIE Liang, et al. Preliminary Experimental Study of Establishing a Lung Development Model with Specific Pathogen Free Preterm Pigs[J]. Journal of Sichuan University (Medical Sciences), 2021, 52(5): 789-793. DOI:

以SPF级早产猪建立肺发育模型的初步实验研究

Preliminary Experimental Study of Establishing a Lung Development Model with Specific Pathogen Free Preterm Pigs

  • 摘要:
      目的  探究在排除高氧、机械通气、药物等医学干预的条件下,建立无特定病原体(specific pathogen free, SPF)级巴马早产猪肺发育动物模型的最佳剖宫产时间。
      方法  选择胎龄113 天(gestational days, GD)、GD107、GD104、GD101和GD98的SPF级巴马猪系母猪分别进行剖宫产,观察早产仔猪存活情况。根据早产猪的一般资料、存活情况和肺石蜡切片HE染色观察结果,寻找最佳的建立SPF级早产猪肺发育模型的剖宫产时间。
      结果  剖宫产7只母猪,共获取仔猪55只,其中产后3 h仍存活的共25只。GD98和GD101的仔猪全部死亡,GD104的仔猪存活7只,存活率为33.33%(7/21),GD107和GD113的仔猪全部存活。GD98的仔猪已具备完整的外形和肺部形状;GD104仔猪的肺部开放度已较大,薄壁型肺泡密度更高;GD107仔猪的肺基本处于开放状态,薄壁肺泡密度基本与正常足月猪相同。
      结论  本研究提示可以利用无医疗干预的GD104 SPF级早产猪建立SPF级早产猪肺发育模型。
     
    Abstract:
      Objective  To determine the best time for conducting cesarean section for the establishment of an animal model of lung development with specific pathogen free (SPF) preterm Bama minipigs under the condition of not making medical interventions such as hyperoxia, mechanical ventilation, or medication.
      Methods  SPF Bama sows at gestational day (GD) 113, GD107, GD104, GD101, and GD98 were selected and cesarean sections were performed. Then, the viability of the preterm piglets were observed. Based on their general data, viability, and paraffin sections stained with hematoxylin and eosin, the best time for performing cesarean section in order to build a SPF preterm pig model of lung development was determined.
      Results  Cesarean sections were performed on a total of 7 sows and 55 piglets were delivered, among which 25 were still alive 3 hours after delivery. Seven piglets of GD104 and all piglets of GD107 and GD113 survived, while piglets of GD98 and GD101 all died. The survival rate of piglets of GD104 was 33.33% (7/21). Piglets of GD98 already possessed fully developed physical appearance and lung shape. Piglets from GD104 had better lung expansion and higher density of thin-walled alveoli. The lungs of GD107 piglets were basically fully expanded, and the density of thin-walled alveoli was almost the same as that of normal full-term piglets.
      Conclusions  Findings of this study suggest that SPF preterm piglets of GD104 with no specific pathogen exposure and no medical intervention can be used to establish a SPF preterm pig model of lung development.
     

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